Gold nanoparticles (AuNPs) with an estimate diameter of 25 nm have been synthesized and characterized using ultraviolet-visible spectroscopy (UV-Vis), dynamic light scattering (DLS) and Zeta potential measurements. AuNPs were coated with D2B, a monoclonal antibody recognizing an extracellular epitope of the human prostate specific membrane antigen (hPSMA). Binding of D2B to PSMA induces endocytosis of the latter, thereby marking PSMA as a docking site for the delivery of therapeutic agents. To attain a stabilized and covalent bond between D2B and AuNPs, the sulthydryl group (SH) in a cysteine ...

Prostate cancer (PCa) is the most frequent cancer malignancy among men and the third leading cause of cancer death in the developed world. To date, the exact molecular mechanism underlying the role of caffeine one of the major coffee constituents on PCa prognosis is not yet unraveled. Caffeine has many antioxidant, anti-inflammatory and anti carcinogenic properties through the modulation of certain inflammatory markers such as IL-6 and TNF-α. The present study was carried out to evaluate the effect of caffeine on PCa cell lines viability, namely PC3 and PC3-PSMA and assessing as well their ...